Perry G. Wang and Weixuan (Eds): Hydrophilic Interaction Liquid Chromatography (HILIC) and Advanced Applications
نویسندگان
چکیده
منابع مشابه
Hydrophilic interaction liquid chromatography (HILIC) in proteomics
In proteomics, nanoflow multidimensional chromatography is now the gold standard for the separation of complex mixtures of peptides as generated by in-solution digestion of whole-cell lysates. Ideally, the different stationary phases used in multidimensional chromatography should provide orthogonal separation characteristics. For this reason, the combination of strong cation exchange chromatogr...
متن کاملHydrophilic interaction liquid chromatography (HILIC)—a powerful separation technique
Hydrophilic interaction liquid chromatography (HILIC) provides an alternative approach to effectively separate small polar compounds on polar stationary phases. The purpose of this work was to review the options for the characterization of HILIC stationary phases and their applications for separations of polar compounds in complex matrices. The characteristics of the hydrophilic stationary phas...
متن کاملA UPLC Method for Analysis of Metformin and Related Substances by Hydrophilic Interaction Chromatography (HILIC)
IN T RO DU C T IO N Metformin hydrochloride is an anti-diabetic drug typically administered orally while treating non-insulin dependant (type 2) diabetes mellitus.1 It is one of the most widely-used oral antidiabetic drugs worldwide, with over 48 million generic formulation prescriptions filled in the United States alone.2 Liquid chromatographic analysis of metformin and related impurities pres...
متن کاملCombining Hydrophilic Interaction Chromatography (HILIC) and Isotope Tagging for Off-Line LC-NMR Applications in Metabolite Analysis
The complementary use of liquid chromatography (LC) and nuclear magnetic resonance (NMR) has shown high utility in a variety of fields. While the significant benefit of spectral simplification can be achieved for the analysis of complex samples, other limitations remain. For example, (1)H LC-NMR suffers from pH dependent chemical shift variations, especially during urine analysis, owing to the ...
متن کاملQuantification of N-hydroxysuccinimide and N-hydroxysulfosuccinimide by hydrophilic interaction chromatography (HILIC)
N-Hydroxysuccinimide (NHS) esters are the most important activated esters used in many different bioconjugation techniques, such as protein labelling by fluorescent dyes and enzymes, surface activation of chromatographic supports, microbeads, nanoparticles, and microarray slides, and also in the chemical synthesis of peptides. Usually, reactions with NHS esters are very reliable and of high yie...
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ژورنال
عنوان ژورنال: Chromatographia
سال: 2012
ISSN: 0009-5893,1612-1112
DOI: 10.1007/s10337-012-2258-9